TY - JOUR
T1 - Carcinoembryonic antigen admittance biosensor based on Au and ZnO nanoparticles using FFT admittance voltammetry
AU - Norouzi, Parviz
AU - Gupta, Vinod Kumar
AU - Faridbod, Farnoush
AU - Pirali-Hamedani, Morteza
AU - Larijani, Bagher
AU - Ganjali, Mohammad Reza
PY - 2011/3/1
Y1 - 2011/3/1
N2 - In this work, a highly sensitive carcinoembryonic antigen fast Fourier transform admittance biosensor is introduced. The proposed biosensor is based on bilayer films of ZnO/Au nanoparticles as an immobilization matrix. These layers are prepared by self-assembly and deposition method on a gold electrode surface, respectively. Carcinoembryonic antibody (anti-CEA) was immobilized on gold nanoparticles and positively charged horseradish peroxidase (HRP) was used to block sites against nonspecific binding. The admittance biosensor was developed based on fast Fourier transform continuous square wave voltammetry, which produces a sensitive, fast (less than 20 s) and reliable response for determination of carcinoembryonic antigen. The technique was applied as a detector in a flow injection system. The admittances reduction current of the biosensor decreases linearly in two concentrations ranges of CEA from 0.1 to 70 ng/mL and from 70 to 200 ng/mL with a detection limit of 0.01 ng/mL in presence of 0.5 mM H2O2 as an eluent solution.
AB - In this work, a highly sensitive carcinoembryonic antigen fast Fourier transform admittance biosensor is introduced. The proposed biosensor is based on bilayer films of ZnO/Au nanoparticles as an immobilization matrix. These layers are prepared by self-assembly and deposition method on a gold electrode surface, respectively. Carcinoembryonic antibody (anti-CEA) was immobilized on gold nanoparticles and positively charged horseradish peroxidase (HRP) was used to block sites against nonspecific binding. The admittance biosensor was developed based on fast Fourier transform continuous square wave voltammetry, which produces a sensitive, fast (less than 20 s) and reliable response for determination of carcinoembryonic antigen. The technique was applied as a detector in a flow injection system. The admittances reduction current of the biosensor decreases linearly in two concentrations ranges of CEA from 0.1 to 70 ng/mL and from 70 to 200 ng/mL with a detection limit of 0.01 ng/mL in presence of 0.5 mM H2O2 as an eluent solution.
UR - http://www.scopus.com/inward/record.url?scp=79952136831&partnerID=8YFLogxK
U2 - 10.1021/ac102270w
DO - 10.1021/ac102270w
M3 - Article
C2 - 21309514
AN - SCOPUS:79952136831
SN - 0003-2700
VL - 83
SP - 1564
EP - 1570
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 5
ER -