Carbon-nanotube-modified electrodes for amplified enzyme-based electrical detection of DNA hybridization

Joseph Wang; Abdel Nasser Kawde; M. Rasul Jan

doi:10.1016/j.bios.2004.06.016

Carbon-nanotube-modified electrodes for amplified enzyme-based electrical detection of DNA hybridization

Joseph Wang^*
, Abdel Nasser Kawde
, M. Rasul Jan

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

82 Scopus citations

Abstract

Carbon-nanotube-modified glassy carbon (CNT/GC) transducers have been developed for enhancing the sensitivity and stability of enzyme-based electrochemical bioassays of DNA hybridization. The amplified signal reflects the interfacial accumulation of phenolic products of the alkaline-phosphatase tracer onto the CNT layer. In particular, chronopotentiometric measurements (following short accumulation periods) offer a substantial enhancement of the response of enzymatically liberated α-naphthol. The CNT modifier also leads to a dramatic improvement in the stability of the amperometric response of α-naphthol. These advantages of CNT/GC electrodes are illustrated from comparison to unmodified glassy carbon electrodes. Factors influencing the adsorptive accumulation of α-naphthol, and the overall performance of the new DNA assay, are assessed and optimized. The attractive performance characteristics of the new multi-amplification electrochemical detection of DNA hybridization are reported in connection to the detection of nucleic acid sequences related to the breast cancer BRCA1 gene.

Original language	English
Pages (from-to)	995-1000
Number of pages	6
Journal	Biosensors and Bioelectronics
Volume	20
Issue number	5
DOIs	https://doi.org/10.1016/j.bios.2004.06.016
State	Published - 15 Nov 2004
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the National Science Foundation (Grant Numbers CHE 0209707 and OCE-332918), US Army Medical Research (Award No. DAMD17-00-1-0366), and NATO (Science for Peace Program). M.R.J. acknowledges a Fulbright fellowship.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Carbon nanotube
DNA hybridization
Enzyme tag
Naphthol

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

Access to Document

10.1016/j.bios.2004.06.016

Cite this

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title = "Carbon-nanotube-modified electrodes for amplified enzyme-based electrical detection of DNA hybridization",

abstract = "Carbon-nanotube-modified glassy carbon (CNT/GC) transducers have been developed for enhancing the sensitivity and stability of enzyme-based electrochemical bioassays of DNA hybridization. The amplified signal reflects the interfacial accumulation of phenolic products of the alkaline-phosphatase tracer onto the CNT layer. In particular, chronopotentiometric measurements (following short accumulation periods) offer a substantial enhancement of the response of enzymatically liberated α-naphthol. The CNT modifier also leads to a dramatic improvement in the stability of the amperometric response of α-naphthol. These advantages of CNT/GC electrodes are illustrated from comparison to unmodified glassy carbon electrodes. Factors influencing the adsorptive accumulation of α-naphthol, and the overall performance of the new DNA assay, are assessed and optimized. The attractive performance characteristics of the new multi-amplification electrochemical detection of DNA hybridization are reported in connection to the detection of nucleic acid sequences related to the breast cancer BRCA1 gene.",

keywords = "Carbon nanotube, DNA hybridization, Enzyme tag, Naphthol",

author = "Joseph Wang and Kawde, \{Abdel Nasser\} and Jan, \{M. Rasul\}",

note = "Funding Information: This work was supported by the National Science Foundation (Grant Numbers CHE 0209707 and OCE-332918), US Army Medical Research (Award No. DAMD17-00-1-0366), and NATO (Science for Peace Program). M.R.J. acknowledges a Fulbright fellowship.",

year = "2004",

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doi = "10.1016/j.bios.2004.06.016",

language = "English",

volume = "20",

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T1 - Carbon-nanotube-modified electrodes for amplified enzyme-based electrical detection of DNA hybridization

AU - Wang, Joseph

AU - Kawde, Abdel Nasser

AU - Jan, M. Rasul

N1 - Funding Information: This work was supported by the National Science Foundation (Grant Numbers CHE 0209707 and OCE-332918), US Army Medical Research (Award No. DAMD17-00-1-0366), and NATO (Science for Peace Program). M.R.J. acknowledges a Fulbright fellowship.

PY - 2004/11/15

Y1 - 2004/11/15

N2 - Carbon-nanotube-modified glassy carbon (CNT/GC) transducers have been developed for enhancing the sensitivity and stability of enzyme-based electrochemical bioassays of DNA hybridization. The amplified signal reflects the interfacial accumulation of phenolic products of the alkaline-phosphatase tracer onto the CNT layer. In particular, chronopotentiometric measurements (following short accumulation periods) offer a substantial enhancement of the response of enzymatically liberated α-naphthol. The CNT modifier also leads to a dramatic improvement in the stability of the amperometric response of α-naphthol. These advantages of CNT/GC electrodes are illustrated from comparison to unmodified glassy carbon electrodes. Factors influencing the adsorptive accumulation of α-naphthol, and the overall performance of the new DNA assay, are assessed and optimized. The attractive performance characteristics of the new multi-amplification electrochemical detection of DNA hybridization are reported in connection to the detection of nucleic acid sequences related to the breast cancer BRCA1 gene.

AB - Carbon-nanotube-modified glassy carbon (CNT/GC) transducers have been developed for enhancing the sensitivity and stability of enzyme-based electrochemical bioassays of DNA hybridization. The amplified signal reflects the interfacial accumulation of phenolic products of the alkaline-phosphatase tracer onto the CNT layer. In particular, chronopotentiometric measurements (following short accumulation periods) offer a substantial enhancement of the response of enzymatically liberated α-naphthol. The CNT modifier also leads to a dramatic improvement in the stability of the amperometric response of α-naphthol. These advantages of CNT/GC electrodes are illustrated from comparison to unmodified glassy carbon electrodes. Factors influencing the adsorptive accumulation of α-naphthol, and the overall performance of the new DNA assay, are assessed and optimized. The attractive performance characteristics of the new multi-amplification electrochemical detection of DNA hybridization are reported in connection to the detection of nucleic acid sequences related to the breast cancer BRCA1 gene.

KW - Carbon nanotube

KW - DNA hybridization

KW - Enzyme tag

KW - Naphthol

UR - https://www.scopus.com/pages/publications/7644228132

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DO - 10.1016/j.bios.2004.06.016

M3 - Article

C2 - 15530796

AN - SCOPUS:7644228132

SN - 0956-5663

VL - 20

SP - 995

EP - 1000

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 5

ER -

Carbon-nanotube-modified electrodes for amplified enzyme-based electrical detection of DNA hybridization

Abstract

Bibliographical note

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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