Brush-like amphoteric poly[isobutylene-alt-(maleic acid)-graft- oligoethyleneamine]/DNA complexes for efficient gene transfectiona

Majad Khan, Goliath Beniah, Nikken Wiradharma, Xin Dong Guo, Yi Yan Yang

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

(Figure Presented) Synthetic gene delivery vectors, especially cationic polymers have attracted enormous attention in recent decades because of their ease of manufacture, targettability, and scaling up. However, certain issues such as high cytotoxicity and low transfection efficiency problems have hampered the advance of nonviral gene delivery. In this study, we designed and synthesized brush-like amphoteric poly[isobutylene-alt-(maleic acid)-graft-oligoethyleneamine] capable of mediating highly efficient gene transfection. The polymers are composed of multiple pendant oligoethyleneimine molecules with alternating carboxylic acid moiety grafted onto poly[isobutylene-alt-(maleic anhydride)]. The polymer formed from pentaethylenehexamine {i.e., poly[isobutylene-alt-(maleic acid)-graft- pentaethylenehexamine)]} was able to condense DNA efficiently into nanoparticles of size around 200 nm with positive zeta potential of about 28-30 mV despite its amphoteric nature. Luciferase expression level and percentage of GFP expressing cells induced by this polymer was higher than those mediated with polyethyleneimine (branched, M̄w 25 kDa) by at least one order of magnitude at their optimal N/P ratios on HEK293, HepG2, and 4T1 cells. In vitro cytotoxicity testing revealed that the polymer/DNA complexes were less cytotoxic than those of PEI, and the viability of the cells after being incubated with the polymer/DNA complexes at the optimal N/P ratios was higher than 85%. This polymer can be a promising gene delivery carrier for gene therapy.

Original languageEnglish
Pages (from-to)1142-1147
Number of pages6
JournalMacromolecular Rapid Communications
Volume31
Issue number13
DOIs
StatePublished - 1 Jul 2010
Externally publishedYes

Keywords

  • Amphoteric
  • Brush-like
  • Gene transfection
  • NMR
  • Polymers
  • Water-soluble

ASJC Scopus subject areas

  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry

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