TY - JOUR
T1 - Bioremediation of lead contaminated environment by Bacillus cereus strain BUK_BCH_BTE2
T2 - Isolation and characterization of the bacterium
AU - Harun, Fatima Abdullahi
AU - Yusuf, Muhammad Rabiú
AU - Usman, Shehu
AU - Shehu, Dayyabu
AU - Babagana, Kamaluddeen
AU - Sufyanu, Aminu Jibril
AU - Jibril, Muhammad Mustapha
AU - Bello, Aliyu Maje
AU - Musa, Kabiru Abubakar
AU - Jagaba, Ahmad Hussaini
AU - Shukor, Mohd Yunus
AU - Yakasai, Hafeez Muhammad
N1 - Publisher Copyright:
© 2023 The Authors
PY - 2023/12
Y1 - 2023/12
N2 - Lead is one of the most toxic heavy metals ever known by man today, which has zero biological role. It is known to cause anemia and affect the nervous and reproductive systems with irreversible effects. Its continual usage leads to its high accumulation polluting the soil and water bodies with devastating health effects. This research, aimed at isolation and characterization of indigenous lead tolerant bacteria from contaminated soil of Anka. Results of Atomic Absorption Spectrophotometry (AAS) showed high concentration of lead (738 mg/kg) of the affected sites above the EPA standard (400 mg/kg). Similarly, a mineral salt media was used to isolate the bacteria following serial dilution. An indigenous bacterial isolate (Ac) with potential to tolerate up to 3000 mg/L Pb(NO3)2 was isolated and molecularly identified based on 16s rRNA sequencing as Bacillus cereus strain BUK_BCH_BTE2 with the accession number MT160412. The isolate was further characterized for its optimum growth and tolerance conditions using one factor at a time (OFAT). Sucrose with an optimum concentration of 5 g/L was the best carbon source for the isolate. The isolate was found to utilize ammonium sulphate as the best nitrogen source at a concentration of 2.5 g/L, with an optimum pH and tempertaure of 7.0 and 37 °C respectively. A concentration of 1000 mg/L Pb(NO3)2 was found to be the optimum concentration for the isolate. The optimum incubation time and inoculum size were found to be 48 hrs and 100 μL respectively. The fact that the isolate could tolerate high Pb(NO3)2 concentration makes it suitable for future bioremediation work involving lead.
AB - Lead is one of the most toxic heavy metals ever known by man today, which has zero biological role. It is known to cause anemia and affect the nervous and reproductive systems with irreversible effects. Its continual usage leads to its high accumulation polluting the soil and water bodies with devastating health effects. This research, aimed at isolation and characterization of indigenous lead tolerant bacteria from contaminated soil of Anka. Results of Atomic Absorption Spectrophotometry (AAS) showed high concentration of lead (738 mg/kg) of the affected sites above the EPA standard (400 mg/kg). Similarly, a mineral salt media was used to isolate the bacteria following serial dilution. An indigenous bacterial isolate (Ac) with potential to tolerate up to 3000 mg/L Pb(NO3)2 was isolated and molecularly identified based on 16s rRNA sequencing as Bacillus cereus strain BUK_BCH_BTE2 with the accession number MT160412. The isolate was further characterized for its optimum growth and tolerance conditions using one factor at a time (OFAT). Sucrose with an optimum concentration of 5 g/L was the best carbon source for the isolate. The isolate was found to utilize ammonium sulphate as the best nitrogen source at a concentration of 2.5 g/L, with an optimum pH and tempertaure of 7.0 and 37 °C respectively. A concentration of 1000 mg/L Pb(NO3)2 was found to be the optimum concentration for the isolate. The optimum incubation time and inoculum size were found to be 48 hrs and 100 μL respectively. The fact that the isolate could tolerate high Pb(NO3)2 concentration makes it suitable for future bioremediation work involving lead.
KW - Bacteria
KW - Heavy metals
KW - Isolation
KW - Lead
KW - Lead-tolerant
UR - https://www.scopus.com/pages/publications/85175694211
U2 - 10.1016/j.cscee.2023.100540
DO - 10.1016/j.cscee.2023.100540
M3 - Article
AN - SCOPUS:85175694211
SN - 2666-0164
VL - 8
JO - Case Studies in Chemical and Environmental Engineering
JF - Case Studies in Chemical and Environmental Engineering
M1 - 100540
ER -
