TY - JOUR
T1 - Bergia ammannioides
T2 - Phytochemical screening, antioxidant activity and radical scavenging effects of its various fractions
AU - Aziz-Ur-Rehman,
AU - Siddiqa, Asia
AU - Abbasi, Muhammad Athar
AU - Riaz, Tauheeda
AU - Hussain, Ghulam
AU - Siddiqui, Sabahat Zahra
AU - Irshad, Misbah
AU - Ajaib, Muhammad
AU - Ahmad, Irshad
PY - 2013
Y1 - 2013
N2 - The methanolic extract of Bergia ammannioides (Henye ex Roth) was dissolved in distilled water and partitioned with n-hexane, chloroform, I ethyl acetate and n-butanol sequentially. The antioxidant potential of all these fractions and remaining aqueous fraction was evaluated by four methods e.g., 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, total antioxidant activity, ferric reducing anti-oxidant power (FRAP) assay and ferric thiocyanate assay along with determination of their total phenolics. Polar fractions showed noteworthy antioxidant potential. The results revealed that ethyl acetate soluble fraction showed highest value of % inhibition of DPPH radical (81.16 ± 0.87 %) at a concentration of 120 μg/mL. The IC 50 of this fraction was 26.65 ± 1.8 μg/mL, relative to ascorbic acid, a reference standard, having IC50 58.8 ± 0.89 μg/mL. It also showed highest total antioxidant activity i.e., 1.142 ± 0.08, highest FRAP value (378.5 ± 0.4 TE μM/mL) as well as highest total phenolic contents i.e., 226.109 ± 0.3 GAE mg/g and highest value of per cent inhibition of lipid peroxidation (54.23 ± 0.57 %) as compared to the studied fractions.
AB - The methanolic extract of Bergia ammannioides (Henye ex Roth) was dissolved in distilled water and partitioned with n-hexane, chloroform, I ethyl acetate and n-butanol sequentially. The antioxidant potential of all these fractions and remaining aqueous fraction was evaluated by four methods e.g., 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, total antioxidant activity, ferric reducing anti-oxidant power (FRAP) assay and ferric thiocyanate assay along with determination of their total phenolics. Polar fractions showed noteworthy antioxidant potential. The results revealed that ethyl acetate soluble fraction showed highest value of % inhibition of DPPH radical (81.16 ± 0.87 %) at a concentration of 120 μg/mL. The IC 50 of this fraction was 26.65 ± 1.8 μg/mL, relative to ascorbic acid, a reference standard, having IC50 58.8 ± 0.89 μg/mL. It also showed highest total antioxidant activity i.e., 1.142 ± 0.08, highest FRAP value (378.5 ± 0.4 TE μM/mL) as well as highest total phenolic contents i.e., 226.109 ± 0.3 GAE mg/g and highest value of per cent inhibition of lipid peroxidation (54.23 ± 0.57 %) as compared to the studied fractions.
KW - Bergia ammannioides
KW - DPPH assay
KW - FRAP value
KW - Total antioxidant activity
KW - Total phenolics
UR - https://www.scopus.com/pages/publications/84883417923
U2 - 10.14233/ajchem.2013.14689
DO - 10.14233/ajchem.2013.14689
M3 - Article
AN - SCOPUS:84883417923
SN - 0970-7077
VL - 25
SP - 7921
EP - 7926
JO - Asian Journal of Chemistry
JF - Asian Journal of Chemistry
IS - 14
ER -