Abstract
We have improved the design of compartmental electrophoresis apparatus. Gel casting has been made very quickly and simply by employing perspex plugs which screw into wax casting cylinders. The apparatus has been automated by connecting an electronic circuitry to a peristaltic pump which keeps the levels and pH of buffers constant in anodic and cathodic chambers. A water jacket has also been added to the apparatus. This vastly improved version of compartmental electrophoresis apparatus could now be successfully used for the separation of noncovalently linked polysaccharides from any protein or enzyme.
| Original language | English |
|---|---|
| Pages (from-to) | 76-77 |
| Number of pages | 2 |
| Journal | Enzyme and Microbial Technology |
| Volume | 22 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1 Jan 1998 |
| Externally published | Yes |
Bibliographical note
Funding Information:We wish to acknowledge the NIBGE Director Dr. Kauser A. Malik for providing research facilities. We are very grateful to NIAB Senior Engineer, Mr. M. Shaukat for fabricating the apparatus. The valuable suggestions of Mr. M.A. Farooqui regarding the design of circuitry are appreciated. This work was financed by PAEC. The technical assistance of G.A. Waseer is also acknowledged.
Keywords
- Automation
- Compartmental electrophoresis
- Electronic circuitry
- Polysaccharides
- Proteins
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology