Application of micro-solid-phase extraction for the determination of persistent organic pollutants in tissue samples

Chanbasha Basheer; Kothandaraman Narasimhan; Meihui Yin; Changqing Zhao; Mahesh Choolani; Hian Kee Lee

doi:10.1016/j.chroma.2007.10.015

Application of micro-solid-phase extraction for the determination of persistent organic pollutants in tissue samples

Chanbasha Basheer
, Kothandaraman Narasimhan
, Meihui Yin
, Changqing Zhao
, Mahesh Choolani
, Hian Kee Lee^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

A novel, simple and efficient method for determining persistent organic pollutants (POPs) in tissue samples has been developed. This technique involves the use of simultaneous microwave-assisted digestion (MAD) and micro-solid-phase extraction (μ-SPE), in which the sorbent is held within a propylene membrane envelope, with gas chromatographic-mass spectrometric (GC-MS) analysis. The POPs studied included eleven organochlorine pesticides and five polychlorinated biphenyl congeners. Optimization of the MAD-μ-SPE parameters was performed. The relative standard deviations (RSDs) of the method ranged from 0.14 to 12.7%. Correlation coefficients up to 0.9999 were obtained across a concentration range of 1.25-50 ng g^-1. The method detection limits for POPs ranged from 0.002 to 0.009 ng g^-1. A preliminary study applying the MAD-μ-SPE procedure to human ovarian cancer tissue showed that it was capable of detecting the presence of a wide spectrum of different POPs in benign and malignant tumors.

Original language	English
Pages (from-to)	358-364
Number of pages	7
Journal	Journal of Chromatography A
Volume	1186
Issue number	1-2
DOIs	https://doi.org/10.1016/j.chroma.2007.10.015
State	Published - 4 Apr 2008
Externally published	Yes

Bibliographical note

Funding Information:
The authors gratefully acknowledge the financial support of this research by the National University of Singapore and the United Nations University, Japan.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Micro-solid phase extraction
Microwave-assisted extraction
Ovarian cancer
Persistent organic pollutants

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/j.chroma.2007.10.015

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@article{9166754d34d54b5a9ada13b4436b4016,

title = "Application of micro-solid-phase extraction for the determination of persistent organic pollutants in tissue samples",

abstract = "A novel, simple and efficient method for determining persistent organic pollutants (POPs) in tissue samples has been developed. This technique involves the use of simultaneous microwave-assisted digestion (MAD) and micro-solid-phase extraction (μ-SPE), in which the sorbent is held within a propylene membrane envelope, with gas chromatographic-mass spectrometric (GC-MS) analysis. The POPs studied included eleven organochlorine pesticides and five polychlorinated biphenyl congeners. Optimization of the MAD-μ-SPE parameters was performed. The relative standard deviations (RSDs) of the method ranged from 0.14 to 12.7\%. Correlation coefficients up to 0.9999 were obtained across a concentration range of 1.25-50 ng g-1. The method detection limits for POPs ranged from 0.002 to 0.009 ng g-1. A preliminary study applying the MAD-μ-SPE procedure to human ovarian cancer tissue showed that it was capable of detecting the presence of a wide spectrum of different POPs in benign and malignant tumors.",

keywords = "Micro-solid phase extraction, Microwave-assisted extraction, Ovarian cancer, Persistent organic pollutants",

author = "Chanbasha Basheer and Kothandaraman Narasimhan and Meihui Yin and Changqing Zhao and Mahesh Choolani and Lee, \{Hian Kee\}",

note = "Funding Information: The authors gratefully acknowledge the financial support of this research by the National University of Singapore and the United Nations University, Japan. ",

year = "2008",

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doi = "10.1016/j.chroma.2007.10.015",

language = "English",

volume = "1186",

pages = "358--364",

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TY - JOUR

T1 - Application of micro-solid-phase extraction for the determination of persistent organic pollutants in tissue samples

AU - Basheer, Chanbasha

AU - Narasimhan, Kothandaraman

AU - Yin, Meihui

AU - Zhao, Changqing

AU - Choolani, Mahesh

AU - Lee, Hian Kee

N1 - Funding Information: The authors gratefully acknowledge the financial support of this research by the National University of Singapore and the United Nations University, Japan.

PY - 2008/4/4

Y1 - 2008/4/4

N2 - A novel, simple and efficient method for determining persistent organic pollutants (POPs) in tissue samples has been developed. This technique involves the use of simultaneous microwave-assisted digestion (MAD) and micro-solid-phase extraction (μ-SPE), in which the sorbent is held within a propylene membrane envelope, with gas chromatographic-mass spectrometric (GC-MS) analysis. The POPs studied included eleven organochlorine pesticides and five polychlorinated biphenyl congeners. Optimization of the MAD-μ-SPE parameters was performed. The relative standard deviations (RSDs) of the method ranged from 0.14 to 12.7%. Correlation coefficients up to 0.9999 were obtained across a concentration range of 1.25-50 ng g-1. The method detection limits for POPs ranged from 0.002 to 0.009 ng g-1. A preliminary study applying the MAD-μ-SPE procedure to human ovarian cancer tissue showed that it was capable of detecting the presence of a wide spectrum of different POPs in benign and malignant tumors.

AB - A novel, simple and efficient method for determining persistent organic pollutants (POPs) in tissue samples has been developed. This technique involves the use of simultaneous microwave-assisted digestion (MAD) and micro-solid-phase extraction (μ-SPE), in which the sorbent is held within a propylene membrane envelope, with gas chromatographic-mass spectrometric (GC-MS) analysis. The POPs studied included eleven organochlorine pesticides and five polychlorinated biphenyl congeners. Optimization of the MAD-μ-SPE parameters was performed. The relative standard deviations (RSDs) of the method ranged from 0.14 to 12.7%. Correlation coefficients up to 0.9999 were obtained across a concentration range of 1.25-50 ng g-1. The method detection limits for POPs ranged from 0.002 to 0.009 ng g-1. A preliminary study applying the MAD-μ-SPE procedure to human ovarian cancer tissue showed that it was capable of detecting the presence of a wide spectrum of different POPs in benign and malignant tumors.

KW - Micro-solid phase extraction

KW - Microwave-assisted extraction

KW - Ovarian cancer

KW - Persistent organic pollutants

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M3 - Article

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AN - SCOPUS:40649116168

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JF - Journal of Chromatography A

IS - 1-2

ER -

Application of micro-solid-phase extraction for the determination of persistent organic pollutants in tissue samples

Abstract

Bibliographical note

UN SDGs

Keywords

ASJC Scopus subject areas

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