Antihyperglycemic effect and phytochemical investigation of Rubia cordifolia (Indian Madder) leaves extract

Muhammad Shafiq Khan; Shahid Aziz; Muhammad Zakryya Khan; Zafar Mahmood Khalid; Muhammad Riaz; Dawood Ahmed; Iftikhar Ali; Nazia Arif; Deiji Wang; Muhammad Sheeraz Ahmad; Nafeesa Zahid

doi:10.1515/chem-2021-0053

Antihyperglycemic effect and phytochemical investigation of Rubia cordifolia (Indian Madder) leaves extract

Muhammad Shafiq Khan^*
, Shahid Aziz
, Muhammad Zakryya Khan^*
, Zafar Mahmood Khalid
, Muhammad Riaz
, Dawood Ahmed
, Iftikhar Ali
, Nazia Arif
, Deiji Wang
, Muhammad Sheeraz Ahmad
, Nafeesa Zahid

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Medicinal plants are used as an important source of medicines in pharmaceutical industry. Rubia cordifolia is widely used to cure diabetes mellitus. Present study was aimed to investigate the antihyperglycemic effects of different fractions of R. cordifolia leaves and to analyze its antioxidant effect and phytochemical composition. Male albino mice were randomly distributed into seven groups (n = 7). Group-I was normal control, group-II was Alloxan (100 mg/kg)-induced diabetic control, and group-III was standard drug (Glibenclamide 0.5 mg/kg)-treated group. Animals in groups IV-VII were treated with n-hexane fraction, ethyl acetate fraction, n-butanol fraction and aqueous fraction of R. cordifolia, orally administered (100 mg/kg) once daily up to 28 days after Alloxan induction, respectively. Methanolic extract (ME) and fractions of R. cordifilia were analyzed for antioxidant activity and quantification of total phenolic content and total flavonoid content. HPLC of ME and most active fractions were performed. The results showed that RCEF (G-V) and RCBF (G-VI) have significantly (P < 0.05) reduced the increased level of glucose as compared to toxicant control group. It was further revealed that EF and BF have higher antioxidant activity (having IC50 34.9, 36.86 (μg/mL)) owing to phenolic and flavonoid identified by HPLC.

Original language	English
Pages (from-to)	586-599
Number of pages	14
Journal	Open Chemistry
Volume	19
Issue number	1
DOIs	https://doi.org/10.1515/chem-2021-0053
State	Published - 1 Jan 2021
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2021 Muhammad Shafiq Khan et al., published by De Gruyter.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Alloxan
Antioxidant
Diabetes mellitus
Pancreas
Polyphenolic compounds
Rubia cordifolia

ASJC Scopus subject areas

General Chemistry
Materials Chemistry

Access to Document

10.1515/chem-2021-0053

Cite this

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title = "Antihyperglycemic effect and phytochemical investigation of Rubia cordifolia (Indian Madder) leaves extract",

abstract = "Medicinal plants are used as an important source of medicines in pharmaceutical industry. Rubia cordifolia is widely used to cure diabetes mellitus. Present study was aimed to investigate the antihyperglycemic effects of different fractions of R. cordifolia leaves and to analyze its antioxidant effect and phytochemical composition. Male albino mice were randomly distributed into seven groups (n = 7). Group-I was normal control, group-II was Alloxan (100 mg/kg)-induced diabetic control, and group-III was standard drug (Glibenclamide 0.5 mg/kg)-treated group. Animals in groups IV-VII were treated with n-hexane fraction, ethyl acetate fraction, n-butanol fraction and aqueous fraction of R. cordifolia, orally administered (100 mg/kg) once daily up to 28 days after Alloxan induction, respectively. Methanolic extract (ME) and fractions of R. cordifilia were analyzed for antioxidant activity and quantification of total phenolic content and total flavonoid content. HPLC of ME and most active fractions were performed. The results showed that RCEF (G-V) and RCBF (G-VI) have significantly (P < 0.05) reduced the increased level of glucose as compared to toxicant control group. It was further revealed that EF and BF have higher antioxidant activity (having IC50 34.9, 36.86 (μg/mL)) owing to phenolic and flavonoid identified by HPLC.",

keywords = "Alloxan, Antioxidant, Diabetes mellitus, Pancreas, Polyphenolic compounds, Rubia cordifolia",

author = "Khan, \{Muhammad Shafiq\} and Shahid Aziz and Khan, \{Muhammad Zakryya\} and Khalid, \{Zafar Mahmood\} and Muhammad Riaz and Dawood Ahmed and Iftikhar Ali and Nazia Arif and Deiji Wang and Ahmad, \{Muhammad Sheeraz\} and Nafeesa Zahid",

note = "Publisher Copyright: {\textcopyright} 2021 Muhammad Shafiq Khan et al., published by De Gruyter.",

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doi = "10.1515/chem-2021-0053",

language = "English",

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T1 - Antihyperglycemic effect and phytochemical investigation of Rubia cordifolia (Indian Madder) leaves extract

AU - Khan, Muhammad Shafiq

AU - Aziz, Shahid

AU - Khan, Muhammad Zakryya

AU - Khalid, Zafar Mahmood

AU - Riaz, Muhammad

AU - Ahmed, Dawood

AU - Ali, Iftikhar

AU - Arif, Nazia

AU - Wang, Deiji

AU - Ahmad, Muhammad Sheeraz

AU - Zahid, Nafeesa

PY - 2021/1/1

Y1 - 2021/1/1

N2 - Medicinal plants are used as an important source of medicines in pharmaceutical industry. Rubia cordifolia is widely used to cure diabetes mellitus. Present study was aimed to investigate the antihyperglycemic effects of different fractions of R. cordifolia leaves and to analyze its antioxidant effect and phytochemical composition. Male albino mice were randomly distributed into seven groups (n = 7). Group-I was normal control, group-II was Alloxan (100 mg/kg)-induced diabetic control, and group-III was standard drug (Glibenclamide 0.5 mg/kg)-treated group. Animals in groups IV-VII were treated with n-hexane fraction, ethyl acetate fraction, n-butanol fraction and aqueous fraction of R. cordifolia, orally administered (100 mg/kg) once daily up to 28 days after Alloxan induction, respectively. Methanolic extract (ME) and fractions of R. cordifilia were analyzed for antioxidant activity and quantification of total phenolic content and total flavonoid content. HPLC of ME and most active fractions were performed. The results showed that RCEF (G-V) and RCBF (G-VI) have significantly (P < 0.05) reduced the increased level of glucose as compared to toxicant control group. It was further revealed that EF and BF have higher antioxidant activity (having IC50 34.9, 36.86 (μg/mL)) owing to phenolic and flavonoid identified by HPLC.

AB - Medicinal plants are used as an important source of medicines in pharmaceutical industry. Rubia cordifolia is widely used to cure diabetes mellitus. Present study was aimed to investigate the antihyperglycemic effects of different fractions of R. cordifolia leaves and to analyze its antioxidant effect and phytochemical composition. Male albino mice were randomly distributed into seven groups (n = 7). Group-I was normal control, group-II was Alloxan (100 mg/kg)-induced diabetic control, and group-III was standard drug (Glibenclamide 0.5 mg/kg)-treated group. Animals in groups IV-VII were treated with n-hexane fraction, ethyl acetate fraction, n-butanol fraction and aqueous fraction of R. cordifolia, orally administered (100 mg/kg) once daily up to 28 days after Alloxan induction, respectively. Methanolic extract (ME) and fractions of R. cordifilia were analyzed for antioxidant activity and quantification of total phenolic content and total flavonoid content. HPLC of ME and most active fractions were performed. The results showed that RCEF (G-V) and RCBF (G-VI) have significantly (P < 0.05) reduced the increased level of glucose as compared to toxicant control group. It was further revealed that EF and BF have higher antioxidant activity (having IC50 34.9, 36.86 (μg/mL)) owing to phenolic and flavonoid identified by HPLC.

KW - Alloxan

KW - Antioxidant

KW - Diabetes mellitus

KW - Pancreas

KW - Polyphenolic compounds

KW - Rubia cordifolia

UR - https://www.scopus.com/pages/publications/85106983152

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DO - 10.1515/chem-2021-0053

M3 - Article

AN - SCOPUS:85106983152

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VL - 19

SP - 586

EP - 599

JO - Open Chemistry

JF - Open Chemistry

IS - 1

ER -

Antihyperglycemic effect and phytochemical investigation of Rubia cordifolia (Indian Madder) leaves extract

Abstract

Bibliographical note

UN SDGs

Keywords

ASJC Scopus subject areas

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