Amplified Electrical Transduction of DNA Hybridization Based on Polymeric Beads Loaded with Multiple Gold Nanoparticle Tags

Abdel Nasser Kawde; Joseph Wang

doi:10.1002/elan.200302924

Amplified Electrical Transduction of DNA Hybridization Based on Polymeric Beads Loaded with Multiple Gold Nanoparticle Tags

Abdel Nasser Kawde^*, Joseph Wang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

106 Scopus citations

Abstract

A new strategy for amplifying particle-based electrical DNA detection based on oligonucleotides functionalized with polymeric beads carrying numerous gold nanoparticle tags is described. The gold-tagged beads were prepared by binding biotinylated metal nanoparticles to streptavidin-coated polystyrene spheres. Such use of carrier-sphere amplification platforms is combined with catalytic enlargement of the multiple gold tags and an ultrasensitive electrochemical stripping detection of the dissolved gold tags. The gold-nanoparticle loaded beads and the resulting DNA-linked assembly were characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Factors affecting the performance have been optimized. Such amplified electrical transduction allows detection of DNA targets down to the 300amol level, and offers great promise for ultrasensitive detection of other biorecogntion events.

Original language	English
Pages (from-to)	101-107
Number of pages	7
Journal	Electroanalysis
Volume	16
Issue number	1-2
DOIs	https://doi.org/10.1002/elan.200302924
State	Published - Jan 2004
Externally published	Yes

Keywords

Amplification
DNA hybridization
Gold nanoparticles
Stripping analysis

ASJC Scopus subject areas

Analytical Chemistry
Electrochemistry

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10.1002/elan.200302924

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title = "Amplified Electrical Transduction of DNA Hybridization Based on Polymeric Beads Loaded with Multiple Gold Nanoparticle Tags",

abstract = "A new strategy for amplifying particle-based electrical DNA detection based on oligonucleotides functionalized with polymeric beads carrying numerous gold nanoparticle tags is described. The gold-tagged beads were prepared by binding biotinylated metal nanoparticles to streptavidin-coated polystyrene spheres. Such use of carrier-sphere amplification platforms is combined with catalytic enlargement of the multiple gold tags and an ultrasensitive electrochemical stripping detection of the dissolved gold tags. The gold-nanoparticle loaded beads and the resulting DNA-linked assembly were characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Factors affecting the performance have been optimized. Such amplified electrical transduction allows detection of DNA targets down to the 300amol level, and offers great promise for ultrasensitive detection of other biorecogntion events.",

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author = "Kawde, {Abdel Nasser} and Joseph Wang",

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journal = "Electroanalysis",

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AU - Kawde, Abdel Nasser

AU - Wang, Joseph

PY - 2004/1

Y1 - 2004/1

N2 - A new strategy for amplifying particle-based electrical DNA detection based on oligonucleotides functionalized with polymeric beads carrying numerous gold nanoparticle tags is described. The gold-tagged beads were prepared by binding biotinylated metal nanoparticles to streptavidin-coated polystyrene spheres. Such use of carrier-sphere amplification platforms is combined with catalytic enlargement of the multiple gold tags and an ultrasensitive electrochemical stripping detection of the dissolved gold tags. The gold-nanoparticle loaded beads and the resulting DNA-linked assembly were characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Factors affecting the performance have been optimized. Such amplified electrical transduction allows detection of DNA targets down to the 300amol level, and offers great promise for ultrasensitive detection of other biorecogntion events.

AB - A new strategy for amplifying particle-based electrical DNA detection based on oligonucleotides functionalized with polymeric beads carrying numerous gold nanoparticle tags is described. The gold-tagged beads were prepared by binding biotinylated metal nanoparticles to streptavidin-coated polystyrene spheres. Such use of carrier-sphere amplification platforms is combined with catalytic enlargement of the multiple gold tags and an ultrasensitive electrochemical stripping detection of the dissolved gold tags. The gold-nanoparticle loaded beads and the resulting DNA-linked assembly were characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Factors affecting the performance have been optimized. Such amplified electrical transduction allows detection of DNA targets down to the 300amol level, and offers great promise for ultrasensitive detection of other biorecogntion events.

KW - Amplification

KW - DNA hybridization

KW - Gold nanoparticles

KW - Stripping analysis

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DO - 10.1002/elan.200302924

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SN - 1040-0397

VL - 16

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EP - 107

JO - Electroanalysis

JF - Electroanalysis

IS - 1-2

ER -

Amplified Electrical Transduction of DNA Hybridization Based on Polymeric Beads Loaded with Multiple Gold Nanoparticle Tags

Abstract

Keywords

ASJC Scopus subject areas

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