Aminoclay-conjugated TiO2 synthesis for simultaneous harvesting and wet-disruption of oleaginous Chlorella sp.

Young Chul Lee; Hyun Uk Lee; Kyubock Lee; Bohwa Kim; So Yeun Lee; Moon Hee Choi; Wasif Farooq; Jin Seok Choi; Ji Yeon Park; Jouhahn Lee; You Kwan Oh; Yun Suk Huh

doi:10.1016/j.cej.2014.02.009

Aminoclay-conjugated TiO₂ synthesis for simultaneous harvesting and wet-disruption of oleaginous Chlorella sp.

Young Chul Lee, Hyun Uk Lee, Kyubock Lee, Bohwa Kim, So Yeun Lee, Moon Hee Choi, Wasif Farooq, Jin Seok Choi, Ji Yeon Park, Jouhahn Lee, You Kwan Oh^*, Yun Suk Huh

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

In microalgae-based biorefinement, the integration of harvesting and lipid extraction steps could reduce the costs of downstream processes. One potential of integrated methods, microalgae harvesting by aminoclay-conjugated TiO₂ has been considered. Therein, aminoclay plays an efficient role in microalgae flocculation and direct cell disruption by TiO₂ photocatalytic performance under UV-light irradiation. In the present study, two TiO₂ photocatalysts, a commercial anatase/rutile bicrystalline (of ~5nm diameter) and anatase/brookite bicrystalline (of ~3.5nm diameter) by sol-gel reaction at room temperature, were distributed uniformly onto an aminoclay matrix by ultrasound-irradiated TiO₂ particles, resulting in aminoclay-conjugated TiO₂ composites. Within 10min, the injection of aminoclay-conjugated TiO₂ into the prepared 1.5g/L-concentration microalgal feedstocks produced an ~85% harvesting efficiency for oleaginous Chlorella sp. KR-1. Subsequently, the harvested wet-microalgae biomass was UV-irradiated at 365nm for 3h, thereby effecting the disruption of ~95% of cells.

Original language	English
Pages (from-to)	143-149
Number of pages	7
Journal	Chemical Engineering Journal
Volume	245
DOIs	https://doi.org/10.1016/j.cej.2014.02.009
State	Published - 1 Jun 2014
Externally published	Yes

Bibliographical note

Funding Information:
This work was conducted under the framework of Research and Development Program of the Korea Institute of Energy Research (KIER) (B4-2434-01), by the Advanced Biomass R&D Center (ABC) of Global Frontier Project funded by the Ministry of Education, Science and Technology (ABC-2012-053880), and by the Korea Institute of Energy Technology Evaluation and Planning (KETEP) and Ministry of Knowledge Economy (MKE) of Korea as a part of the Project of “Process demonstration for bioconversion of CO 2 to high-valued biomaterials using microalgae” (2012-T-100201516) in “Energy Efficiency and Resources R&D project”. and by a grant from the New & Renewable Energy Technology Development Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) funded by the Korean MKE (No. 20123010090010). We thank Jin Seok Choi of the KAIST Research Analysis Center for the TEM imaging and its analysis, as well as Dr. Ramasamy Praveenkumar of KIER for the optical and fluorescence analysis of Chlorella sp. KR-1.

Keywords

Aminoclay
Biodiesel
Biorefinement
Extraction
Harvesting
TiO

ASJC Scopus subject areas

General Chemistry
Environmental Chemistry
General Chemical Engineering
Industrial and Manufacturing Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.cej.2014.02.009

Cite this

@article{b81f59dd37bc4defaa422a23a67ccf12,

title = "Aminoclay-conjugated TiO2 synthesis for simultaneous harvesting and wet-disruption of oleaginous Chlorella sp.",

abstract = "In microalgae-based biorefinement, the integration of harvesting and lipid extraction steps could reduce the costs of downstream processes. One potential of integrated methods, microalgae harvesting by aminoclay-conjugated TiO2 has been considered. Therein, aminoclay plays an efficient role in microalgae flocculation and direct cell disruption by TiO2 photocatalytic performance under UV-light irradiation. In the present study, two TiO2 photocatalysts, a commercial anatase/rutile bicrystalline (of \textasciitilde{}5nm diameter) and anatase/brookite bicrystalline (of \textasciitilde{}3.5nm diameter) by sol-gel reaction at room temperature, were distributed uniformly onto an aminoclay matrix by ultrasound-irradiated TiO2 particles, resulting in aminoclay-conjugated TiO2 composites. Within 10min, the injection of aminoclay-conjugated TiO2 into the prepared 1.5g/L-concentration microalgal feedstocks produced an \textasciitilde{}85\% harvesting efficiency for oleaginous Chlorella sp. KR-1. Subsequently, the harvested wet-microalgae biomass was UV-irradiated at 365nm for 3h, thereby effecting the disruption of \textasciitilde{}95\% of cells.",

keywords = "Aminoclay, Biodiesel, Biorefinement, Extraction, Harvesting, TiO",

author = "Lee, \{Young Chul\} and Lee, \{Hyun Uk\} and Kyubock Lee and Bohwa Kim and Lee, \{So Yeun\} and Choi, \{Moon Hee\} and Wasif Farooq and Choi, \{Jin Seok\} and Park, \{Ji Yeon\} and Jouhahn Lee and Oh, \{You Kwan\} and Huh, \{Yun Suk\}",

note = "Funding Information: This work was conducted under the framework of Research and Development Program of the Korea Institute of Energy Research (KIER) (B4-2434-01), by the Advanced Biomass R\&D Center (ABC) of Global Frontier Project funded by the Ministry of Education, Science and Technology (ABC-2012-053880), and by the Korea Institute of Energy Technology Evaluation and Planning (KETEP) and Ministry of Knowledge Economy (MKE) of Korea as a part of the Project of “Process demonstration for bioconversion of CO 2 to high-valued biomaterials using microalgae” (2012-T-100201516) in “Energy Efficiency and Resources R\&D project”. and by a grant from the New \& Renewable Energy Technology Development Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) funded by the Korean MKE (No. 20123010090010). We thank Jin Seok Choi of the KAIST Research Analysis Center for the TEM imaging and its analysis, as well as Dr. Ramasamy Praveenkumar of KIER for the optical and fluorescence analysis of Chlorella sp. KR-1. ",

year = "2014",

month = jun,

day = "1",

doi = "10.1016/j.cej.2014.02.009",

language = "English",

volume = "245",

pages = "143--149",

journal = "Chemical Engineering Journal",

issn = "1385-8947",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Aminoclay-conjugated TiO2 synthesis for simultaneous harvesting and wet-disruption of oleaginous Chlorella sp.

AU - Lee, Young Chul

AU - Lee, Hyun Uk

AU - Lee, Kyubock

AU - Kim, Bohwa

AU - Lee, So Yeun

AU - Choi, Moon Hee

AU - Farooq, Wasif

AU - Choi, Jin Seok

AU - Park, Ji Yeon

AU - Lee, Jouhahn

AU - Oh, You Kwan

AU - Huh, Yun Suk

N1 - Funding Information: This work was conducted under the framework of Research and Development Program of the Korea Institute of Energy Research (KIER) (B4-2434-01), by the Advanced Biomass R&D Center (ABC) of Global Frontier Project funded by the Ministry of Education, Science and Technology (ABC-2012-053880), and by the Korea Institute of Energy Technology Evaluation and Planning (KETEP) and Ministry of Knowledge Economy (MKE) of Korea as a part of the Project of “Process demonstration for bioconversion of CO 2 to high-valued biomaterials using microalgae” (2012-T-100201516) in “Energy Efficiency and Resources R&D project”. and by a grant from the New & Renewable Energy Technology Development Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) funded by the Korean MKE (No. 20123010090010). We thank Jin Seok Choi of the KAIST Research Analysis Center for the TEM imaging and its analysis, as well as Dr. Ramasamy Praveenkumar of KIER for the optical and fluorescence analysis of Chlorella sp. KR-1.

PY - 2014/6/1

Y1 - 2014/6/1

N2 - In microalgae-based biorefinement, the integration of harvesting and lipid extraction steps could reduce the costs of downstream processes. One potential of integrated methods, microalgae harvesting by aminoclay-conjugated TiO2 has been considered. Therein, aminoclay plays an efficient role in microalgae flocculation and direct cell disruption by TiO2 photocatalytic performance under UV-light irradiation. In the present study, two TiO2 photocatalysts, a commercial anatase/rutile bicrystalline (of ~5nm diameter) and anatase/brookite bicrystalline (of ~3.5nm diameter) by sol-gel reaction at room temperature, were distributed uniformly onto an aminoclay matrix by ultrasound-irradiated TiO2 particles, resulting in aminoclay-conjugated TiO2 composites. Within 10min, the injection of aminoclay-conjugated TiO2 into the prepared 1.5g/L-concentration microalgal feedstocks produced an ~85% harvesting efficiency for oleaginous Chlorella sp. KR-1. Subsequently, the harvested wet-microalgae biomass was UV-irradiated at 365nm for 3h, thereby effecting the disruption of ~95% of cells.

AB - In microalgae-based biorefinement, the integration of harvesting and lipid extraction steps could reduce the costs of downstream processes. One potential of integrated methods, microalgae harvesting by aminoclay-conjugated TiO2 has been considered. Therein, aminoclay plays an efficient role in microalgae flocculation and direct cell disruption by TiO2 photocatalytic performance under UV-light irradiation. In the present study, two TiO2 photocatalysts, a commercial anatase/rutile bicrystalline (of ~5nm diameter) and anatase/brookite bicrystalline (of ~3.5nm diameter) by sol-gel reaction at room temperature, were distributed uniformly onto an aminoclay matrix by ultrasound-irradiated TiO2 particles, resulting in aminoclay-conjugated TiO2 composites. Within 10min, the injection of aminoclay-conjugated TiO2 into the prepared 1.5g/L-concentration microalgal feedstocks produced an ~85% harvesting efficiency for oleaginous Chlorella sp. KR-1. Subsequently, the harvested wet-microalgae biomass was UV-irradiated at 365nm for 3h, thereby effecting the disruption of ~95% of cells.

KW - Aminoclay

KW - Biodiesel

KW - Biorefinement

KW - Extraction

KW - Harvesting

KW - TiO

UR - https://www.scopus.com/pages/publications/84894660735

U2 - 10.1016/j.cej.2014.02.009

DO - 10.1016/j.cej.2014.02.009

M3 - Article

AN - SCOPUS:84894660735

SN - 1385-8947

VL - 245

SP - 143

EP - 149

JO - Chemical Engineering Journal

JF - Chemical Engineering Journal

ER -