Acid and enzyme hydrolysis to convert pretreated lignocellulosic materials into glucose for ethanol production

Waleed K. El-Zawawy; Maha M. Ibrahim; Yasser R. Abdel-Fattah; Nadia A. Soliman; Morsi M. Mahmoud

doi:10.1016/j.carbpol.2010.12.022

Acid and enzyme hydrolysis to convert pretreated lignocellulosic materials into glucose for ethanol production

Waleed K. El-Zawawy
, Maha M. Ibrahim
, Yasser R. Abdel-Fattah
, Nadia A. Soliman
, Morsi M. Mahmoud

Research output: Contribution to journal › Article › peer-review

128 Scopus citations

Abstract

The current research investigates the use of acid and enzyme hydrolysis to produce glucose from pretreated rice straw, banana plant waste and corn cob, as a lignocellulosic materials, to be a source for ethanol production. The agricultural biomasses were first tested, then a laboratory experimental set-up was designed in order to perform the necessary conversions. The biomass materials were characterized to contain 57.46-85.28% holocellulose and 14.55-26.12% lignin. Conversion of the cellulose to glucose was achieved by pre-treatment method for the agricultural residues first applying chemical pulping and steam explosion method as well as microwave treatment then followed by two processes, namely acid hydrolysis and enzyme hydrolysis. Sulfuric acid, 5%, was used in acid hydrolysis and Trichoderma reesei cellulases in enzyme hydrolysis. These experiments demonstrated that glucose concentration differs according to the type of pre-treatment and type of hydrolysis. Conversion of the glucose to ethanol during fermentation was accomplished by the action of yeasts from Saccharomyces cerevisiae. Ethanol production in the culture sample was monitored using gas chromatography. The results indicate that ethanol can be made from the above mentioned residues in a different yield according to the pre-treatment and the glucose produced from the hydrolysis method.

Original language	English
Pages (from-to)	865-871
Number of pages	7
Journal	Carbohydrate Polymers
Volume	84
Issue number	3
DOIs	https://doi.org/10.1016/j.carbpol.2010.12.022
State	Published - 17 Mar 2011
Externally published	Yes

Bibliographical note

Funding Information:
The authors acknowledge the Science and Technology Development Fund (STDF) for supporting and funding the research through a funding project entitled: economic conversion of agriculture products into ethanol and products of industrial significance (ID: 212). Also, the authors acknowledge both Prof. Foster Agblevor and Bob Wright from Virginia PolyTechnic Institute and State University, VA, USA , for helping the corresponding author on conducting the steam explosion experiments.

Keywords

Acid hydrolysis
Agricultural biomass
Enzyme hydrolysis
Ethanol production
Glucose concentration
Pre-treatment

ASJC Scopus subject areas

Organic Chemistry
Polymers and Plastics
Materials Chemistry

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10.1016/j.carbpol.2010.12.022

Cite this

@article{a6870e21e67a4ce48866cd0c871fea8e,

title = "Acid and enzyme hydrolysis to convert pretreated lignocellulosic materials into glucose for ethanol production",

abstract = "The current research investigates the use of acid and enzyme hydrolysis to produce glucose from pretreated rice straw, banana plant waste and corn cob, as a lignocellulosic materials, to be a source for ethanol production. The agricultural biomasses were first tested, then a laboratory experimental set-up was designed in order to perform the necessary conversions. The biomass materials were characterized to contain 57.46-85.28\% holocellulose and 14.55-26.12\% lignin. Conversion of the cellulose to glucose was achieved by pre-treatment method for the agricultural residues first applying chemical pulping and steam explosion method as well as microwave treatment then followed by two processes, namely acid hydrolysis and enzyme hydrolysis. Sulfuric acid, 5\%, was used in acid hydrolysis and Trichoderma reesei cellulases in enzyme hydrolysis. These experiments demonstrated that glucose concentration differs according to the type of pre-treatment and type of hydrolysis. Conversion of the glucose to ethanol during fermentation was accomplished by the action of yeasts from Saccharomyces cerevisiae. Ethanol production in the culture sample was monitored using gas chromatography. The results indicate that ethanol can be made from the above mentioned residues in a different yield according to the pre-treatment and the glucose produced from the hydrolysis method.",

keywords = "Acid hydrolysis, Agricultural biomass, Enzyme hydrolysis, Ethanol production, Glucose concentration, Pre-treatment",

author = "El-Zawawy, \{Waleed K.\} and Ibrahim, \{Maha M.\} and Abdel-Fattah, \{Yasser R.\} and Soliman, \{Nadia A.\} and Mahmoud, \{Morsi M.\}",

note = "Funding Information: The authors acknowledge the Science and Technology Development Fund (STDF) for supporting and funding the research through a funding project entitled: economic conversion of agriculture products into ethanol and products of industrial significance (ID: 212). Also, the authors acknowledge both Prof. Foster Agblevor and Bob Wright from Virginia PolyTechnic Institute and State University, VA, USA , for helping the corresponding author on conducting the steam explosion experiments. ",

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doi = "10.1016/j.carbpol.2010.12.022",

language = "English",

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journal = "Carbohydrate Polymers",

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T1 - Acid and enzyme hydrolysis to convert pretreated lignocellulosic materials into glucose for ethanol production

AU - El-Zawawy, Waleed K.

AU - Ibrahim, Maha M.

AU - Abdel-Fattah, Yasser R.

AU - Soliman, Nadia A.

AU - Mahmoud, Morsi M.

N1 - Funding Information: The authors acknowledge the Science and Technology Development Fund (STDF) for supporting and funding the research through a funding project entitled: economic conversion of agriculture products into ethanol and products of industrial significance (ID: 212). Also, the authors acknowledge both Prof. Foster Agblevor and Bob Wright from Virginia PolyTechnic Institute and State University, VA, USA , for helping the corresponding author on conducting the steam explosion experiments.

PY - 2011/3/17

Y1 - 2011/3/17

N2 - The current research investigates the use of acid and enzyme hydrolysis to produce glucose from pretreated rice straw, banana plant waste and corn cob, as a lignocellulosic materials, to be a source for ethanol production. The agricultural biomasses were first tested, then a laboratory experimental set-up was designed in order to perform the necessary conversions. The biomass materials were characterized to contain 57.46-85.28% holocellulose and 14.55-26.12% lignin. Conversion of the cellulose to glucose was achieved by pre-treatment method for the agricultural residues first applying chemical pulping and steam explosion method as well as microwave treatment then followed by two processes, namely acid hydrolysis and enzyme hydrolysis. Sulfuric acid, 5%, was used in acid hydrolysis and Trichoderma reesei cellulases in enzyme hydrolysis. These experiments demonstrated that glucose concentration differs according to the type of pre-treatment and type of hydrolysis. Conversion of the glucose to ethanol during fermentation was accomplished by the action of yeasts from Saccharomyces cerevisiae. Ethanol production in the culture sample was monitored using gas chromatography. The results indicate that ethanol can be made from the above mentioned residues in a different yield according to the pre-treatment and the glucose produced from the hydrolysis method.

AB - The current research investigates the use of acid and enzyme hydrolysis to produce glucose from pretreated rice straw, banana plant waste and corn cob, as a lignocellulosic materials, to be a source for ethanol production. The agricultural biomasses were first tested, then a laboratory experimental set-up was designed in order to perform the necessary conversions. The biomass materials were characterized to contain 57.46-85.28% holocellulose and 14.55-26.12% lignin. Conversion of the cellulose to glucose was achieved by pre-treatment method for the agricultural residues first applying chemical pulping and steam explosion method as well as microwave treatment then followed by two processes, namely acid hydrolysis and enzyme hydrolysis. Sulfuric acid, 5%, was used in acid hydrolysis and Trichoderma reesei cellulases in enzyme hydrolysis. These experiments demonstrated that glucose concentration differs according to the type of pre-treatment and type of hydrolysis. Conversion of the glucose to ethanol during fermentation was accomplished by the action of yeasts from Saccharomyces cerevisiae. Ethanol production in the culture sample was monitored using gas chromatography. The results indicate that ethanol can be made from the above mentioned residues in a different yield according to the pre-treatment and the glucose produced from the hydrolysis method.

KW - Acid hydrolysis

KW - Agricultural biomass

KW - Enzyme hydrolysis

KW - Ethanol production

KW - Glucose concentration

KW - Pre-treatment

UR - https://www.scopus.com/pages/publications/79951681988

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M3 - Article

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VL - 84

SP - 865

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JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

IS - 3

ER -

Acid and enzyme hydrolysis to convert pretreated lignocellulosic materials into glucose for ethanol production

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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