Acellular human amniotic membrane: A safe and stable matrix for corneal stromal regeneration

Khosrow Jadidi; Mahsa Fallah Tafti; Mohamad Hadi Dianat; Shokoofeh Rafati; Mohsen Ghiasi; Naghmeh Rafati; Farrokh Modarresi; Esmaeel Sharifi; Anwarul Hasan; Hossein Aghamollaei; Pooyan Makvandi

doi:10.1016/j.colsurfa.2024.133745

Acellular human amniotic membrane: A safe and stable matrix for corneal stromal regeneration

Khosrow Jadidi
, Mahsa Fallah Tafti
, Mohamad Hadi Dianat
, Shokoofeh Rafati
, Mohsen Ghiasi
, Naghmeh Rafati
, Farrokh Modarresi
, Esmaeel Sharifi
, Anwarul Hasan
, Hossein Aghamollaei^*
, Pooyan Makvandi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Amniotic membrane (AM) transplantation is used as a safe and effective method to treat a variety of ocular complications. On the ocular surface, this membrane degrades within a few days. This study investigated the safety and long-term stability of human amniotic membrane (HAM) grafts in the corneal stroma. Three rabbits with healthy eyes underwent unilateral stromal pocketing with implantation of decellularized HAM (dHAM). Examinations of graft bio-integration were performed by corneal imaging using slit lamp and optical coherence tomography (OCT) at 1 week, 3 months, and 9 months. The thickness of the thickest part of the amniotic membrane was 187 μm at the first follow-up, 184 μm at 3 months, and 166 μm at 9 months. Histological evaluation was performed after the last follow-up. The results showed no clinical complications, such as opacity, neovascularization, infection, or rejection. The thickness of the grafts remained stable over time. Histopathological analyses showed the grafts were integrated into the corneal stroma without any inflammation. This study indicated that HAM was a naturally safe, stable, and compatible matrix in the corneal stroma and was considered a promising candidate for corneal stromal regeneration.

Original language	English
Article number	133745
Journal	Colloids and Surfaces A: Physicochemical and Engineering Aspects
Volume	690
DOIs	https://doi.org/10.1016/j.colsurfa.2024.133745
State	Published - 5 Jun 2024
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2024 Elsevier B.V.

Keywords

Amniotic membrane transplantation
Corneal tissue
Tissue engineering

ASJC Scopus subject areas

Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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10.1016/j.colsurfa.2024.133745

Cite this

Jadidi, K., Tafti, M. F., Dianat, M. H., Rafati, S., Ghiasi, M., Rafati, N., Modarresi, F., Sharifi, E., Hasan, A., Aghamollaei, H., & Makvandi, P. (2024). Acellular human amniotic membrane: A safe and stable matrix for corneal stromal regeneration. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 690, Article 133745. https://doi.org/10.1016/j.colsurfa.2024.133745

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title = "Acellular human amniotic membrane: A safe and stable matrix for corneal stromal regeneration",

abstract = "Amniotic membrane (AM) transplantation is used as a safe and effective method to treat a variety of ocular complications. On the ocular surface, this membrane degrades within a few days. This study investigated the safety and long-term stability of human amniotic membrane (HAM) grafts in the corneal stroma. Three rabbits with healthy eyes underwent unilateral stromal pocketing with implantation of decellularized HAM (dHAM). Examinations of graft bio-integration were performed by corneal imaging using slit lamp and optical coherence tomography (OCT) at 1 week, 3 months, and 9 months. The thickness of the thickest part of the amniotic membrane was 187 μm at the first follow-up, 184 μm at 3 months, and 166 μm at 9 months. Histological evaluation was performed after the last follow-up. The results showed no clinical complications, such as opacity, neovascularization, infection, or rejection. The thickness of the grafts remained stable over time. Histopathological analyses showed the grafts were integrated into the corneal stroma without any inflammation. This study indicated that HAM was a naturally safe, stable, and compatible matrix in the corneal stroma and was considered a promising candidate for corneal stromal regeneration.",

keywords = "Amniotic membrane transplantation, Corneal tissue, Tissue engineering",

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doi = "10.1016/j.colsurfa.2024.133745",

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T1 - Acellular human amniotic membrane

T2 - A safe and stable matrix for corneal stromal regeneration

AU - Jadidi, Khosrow

AU - Tafti, Mahsa Fallah

AU - Dianat, Mohamad Hadi

AU - Rafati, Shokoofeh

AU - Ghiasi, Mohsen

AU - Rafati, Naghmeh

AU - Modarresi, Farrokh

AU - Sharifi, Esmaeel

AU - Hasan, Anwarul

AU - Aghamollaei, Hossein

AU - Makvandi, Pooyan

PY - 2024/6/5

Y1 - 2024/6/5

N2 - Amniotic membrane (AM) transplantation is used as a safe and effective method to treat a variety of ocular complications. On the ocular surface, this membrane degrades within a few days. This study investigated the safety and long-term stability of human amniotic membrane (HAM) grafts in the corneal stroma. Three rabbits with healthy eyes underwent unilateral stromal pocketing with implantation of decellularized HAM (dHAM). Examinations of graft bio-integration were performed by corneal imaging using slit lamp and optical coherence tomography (OCT) at 1 week, 3 months, and 9 months. The thickness of the thickest part of the amniotic membrane was 187 μm at the first follow-up, 184 μm at 3 months, and 166 μm at 9 months. Histological evaluation was performed after the last follow-up. The results showed no clinical complications, such as opacity, neovascularization, infection, or rejection. The thickness of the grafts remained stable over time. Histopathological analyses showed the grafts were integrated into the corneal stroma without any inflammation. This study indicated that HAM was a naturally safe, stable, and compatible matrix in the corneal stroma and was considered a promising candidate for corneal stromal regeneration.

AB - Amniotic membrane (AM) transplantation is used as a safe and effective method to treat a variety of ocular complications. On the ocular surface, this membrane degrades within a few days. This study investigated the safety and long-term stability of human amniotic membrane (HAM) grafts in the corneal stroma. Three rabbits with healthy eyes underwent unilateral stromal pocketing with implantation of decellularized HAM (dHAM). Examinations of graft bio-integration were performed by corneal imaging using slit lamp and optical coherence tomography (OCT) at 1 week, 3 months, and 9 months. The thickness of the thickest part of the amniotic membrane was 187 μm at the first follow-up, 184 μm at 3 months, and 166 μm at 9 months. Histological evaluation was performed after the last follow-up. The results showed no clinical complications, such as opacity, neovascularization, infection, or rejection. The thickness of the grafts remained stable over time. Histopathological analyses showed the grafts were integrated into the corneal stroma without any inflammation. This study indicated that HAM was a naturally safe, stable, and compatible matrix in the corneal stroma and was considered a promising candidate for corneal stromal regeneration.

KW - Amniotic membrane transplantation

KW - Corneal tissue

KW - Tissue engineering

UR - https://www.scopus.com/pages/publications/85189093242

U2 - 10.1016/j.colsurfa.2024.133745

DO - 10.1016/j.colsurfa.2024.133745

M3 - Article

AN - SCOPUS:85189093242

SN - 0927-7757

VL - 690

JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

M1 - 133745

ER -

Acellular human amniotic membrane: A safe and stable matrix for corneal stromal regeneration

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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