A 1H nmr study of the interaction of aurothiomalate ("Myocrisin") with human red blood cells in vitro

Gabriel Otiko; M. Tahir Razi; Peter J. Sadler; Anvarhusein A. Isab; Dallas L. Rabenstein

doi:10.1016/0162-0134(83)85027-2

A ¹H nmr study of the interaction of aurothiomalate ("Myocrisin") with human red blood cells in vitro

Gabriel Otiko^*, M. Tahir Razi, Peter J. Sadler, Anvarhusein A. Isab, Dallas L. Rabenstein

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

The results of ¹H spin-echo Fourier transform (SEFT) nuclear magnetic resonance (nmr) experiments suggest that some aurothiomalate binds intracellular glutathione (GSH) when added to suspensions of red cells in vitro. When added to red cell lysates, a specific binding of gold to cysteine of GSH is observed together with release of thiomalate. Gold binding to GSH can be reversed by addition of dimercaptopropanol sulfonate. Spectra are compared to those of an aurothiomalate-GSH model system. The relationship of these findings to the mechanism of action of Myocrisin is discussed.

Original language	English
Pages (from-to)	227-235
Number of pages	9
Journal	Journal of Inorganic Biochemistry
Volume	19
Issue number	3
DOIs	https://doi.org/10.1016/0162-0134(83)85027-2
State	Published - Nov 1983
Externally published	Yes

Bibliographical note

Funding Information:
P.J.S. thanks the Arthritis and Rheumatism Council, Smith Kline and French Laboratories, the SERC, Medical Research Council (MRC) and University of London Intercollegiate Research Service for support. D.L.R. thanks the National Science and Engineering Research Council of Canada for support. A.A.I. was supported&y a Postdoctoral Fellowship from the Alberta Herftage Foundation for Medical Research.

ASJC Scopus subject areas

Biochemistry
Inorganic Chemistry

Access to Document

10.1016/0162-0134(83)85027-2

Cite this

@article{771d12fc9793482ab7b9c56d6bdf0bb6,

title = "A 1H nmr study of the interaction of aurothiomalate ({"}Myocrisin{"}) with human red blood cells in vitro",

abstract = "The results of 1H spin-echo Fourier transform (SEFT) nuclear magnetic resonance (nmr) experiments suggest that some aurothiomalate binds intracellular glutathione (GSH) when added to suspensions of red cells in vitro. When added to red cell lysates, a specific binding of gold to cysteine of GSH is observed together with release of thiomalate. Gold binding to GSH can be reversed by addition of dimercaptopropanol sulfonate. Spectra are compared to those of an aurothiomalate-GSH model system. The relationship of these findings to the mechanism of action of Myocrisin is discussed.",

author = "Gabriel Otiko and Razi, \{M. Tahir\} and Sadler, \{Peter J.\} and Isab, \{Anvarhusein A.\} and Rabenstein, \{Dallas L.\}",

note = "Funding Information: P.J.S. thanks the Arthritis and Rheumatism Council, Smith Kline and French Laboratories, the SERC, Medical Research Council (MRC) and University of London Intercollegiate Research Service for support. D.L.R. thanks the National Science and Engineering Research Council of Canada for support. A.A.I. was supported\&y a Postdoctoral Fellowship from the Alberta Herftage Foundation for Medical Research.",

year = "1983",

month = nov,

doi = "10.1016/0162-0134(83)85027-2",

language = "English",

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journal = "Journal of Inorganic Biochemistry",

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T1 - A 1H nmr study of the interaction of aurothiomalate ("Myocrisin") with human red blood cells in vitro

AU - Otiko, Gabriel

AU - Razi, M. Tahir

AU - Sadler, Peter J.

AU - Isab, Anvarhusein A.

AU - Rabenstein, Dallas L.

N1 - Funding Information: P.J.S. thanks the Arthritis and Rheumatism Council, Smith Kline and French Laboratories, the SERC, Medical Research Council (MRC) and University of London Intercollegiate Research Service for support. D.L.R. thanks the National Science and Engineering Research Council of Canada for support. A.A.I. was supported&y a Postdoctoral Fellowship from the Alberta Herftage Foundation for Medical Research.

PY - 1983/11

Y1 - 1983/11

N2 - The results of 1H spin-echo Fourier transform (SEFT) nuclear magnetic resonance (nmr) experiments suggest that some aurothiomalate binds intracellular glutathione (GSH) when added to suspensions of red cells in vitro. When added to red cell lysates, a specific binding of gold to cysteine of GSH is observed together with release of thiomalate. Gold binding to GSH can be reversed by addition of dimercaptopropanol sulfonate. Spectra are compared to those of an aurothiomalate-GSH model system. The relationship of these findings to the mechanism of action of Myocrisin is discussed.

AB - The results of 1H spin-echo Fourier transform (SEFT) nuclear magnetic resonance (nmr) experiments suggest that some aurothiomalate binds intracellular glutathione (GSH) when added to suspensions of red cells in vitro. When added to red cell lysates, a specific binding of gold to cysteine of GSH is observed together with release of thiomalate. Gold binding to GSH can be reversed by addition of dimercaptopropanol sulfonate. Spectra are compared to those of an aurothiomalate-GSH model system. The relationship of these findings to the mechanism of action of Myocrisin is discussed.

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U2 - 10.1016/0162-0134(83)85027-2

DO - 10.1016/0162-0134(83)85027-2

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