A novel synthesis of non-aggregated spinel nickel ferrite nanosheets for developing non-enzymatic reactive oxygen species sensor in biological samples

Herein, the synthesis of novel non-aggregated spinel nickel ferrite, NiFe₂O₄ nanosheets (NiFe₂O₄ NSs) and its application towards the selective electrocatalytic reduction of hydrogen peroxide are described. Initially, NiFe₂O₄ NSs is synthesized by one-step hydrothermal approach, and numerous characterizations deliberately explain the compound's composition and structure. Finally, the NiFe₂O₄ NSs underwent direct non-enzymatic electrochemistry and succeeded, it as mimicking Horseradish Peroxidase properties. The significance of non-aggregated NiFe₂O₄ NSs together with good electrocatalytic properties leads the material to the platform for electrochemical sensors. Moreover, NiFe₂O₄ NSs is fabricated and validated as an enzyme-free biosensor for the sensitive detection of H₂O₂. The demonstrated sensor revealed excellent detection of H₂O₂ with the pico-molar detection limit (12.4 pM), and also it offered good analytical parameters with more extensive linear range and higher sensitivity. Likewise, the non-enzymatic biosensor annexes good durability, reproducibility, and selectivity towards the determination of H₂O₂. Due to the nourishing capacity of the prepared NiFe₂O₄ NSs, it is employed for the enzyme-free detection of H₂O₂ in human blood and rat brain serum samples.