A Novel DNA biosensor based on a pencil graphite electrode modified with polypyrrole/functionalized multiwalled carbon nanotubes for determination of 6-mercaptopurine anticancer drug

Hassan Karimi-Maleh; Fahimeh Tahernejad-Javazmi; Necip Atar; Mehmet Lütfi Yola; Vinod Kumar Gupta; Ali A. Ensafi

doi:10.1021/ie504438z

A Novel DNA biosensor based on a pencil graphite electrode modified with polypyrrole/functionalized multiwalled carbon nanotubes for determination of 6-mercaptopurine anticancer drug

Hassan Karimi-Maleh^*
, Fahimeh Tahernejad-Javazmi
, Necip Atar
, Mehmet Lütfi Yola
, Vinod Kumar Gupta
, Ali A. Ensafi

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

419 Scopus citations

Abstract

A novel and sensitive biosensor employing immobilized DNA on a pencil graphite electrode modified with polypyrrole/functionalized multiwalled carbon nanotubes for the determination of 6-mercaptopurine (6-MP) is presented. In the first step, we modified the pencil graphite surface with polypyrrole and functionalized multiwalled carbon nanotubes (MWCNT/COOH). The developed electrode was characterized by scanning electron microscopy, atomic force microscopy, reflection-absorption infrared spectroscopy, X-ray photoelectron spectroscopy, and electrochemical impedance spectroscopy. In the other step, we used decreases in the oxidation responses of guanine and adenine as a sign of the interaction of 6-MP with salmon sperm double-stranded DNA using differential pulse voltammetry. The signal of guanine oxidation was linear with respect to the 6-MP concentration in the range of 0.2-100 μmol L^-1 with a detection limit of 0.08 μmol L^-1. The modified electrode was utilized for the determination of 6-MP in real samples.

Original language	English
Pages (from-to)	3634-3639
Number of pages	6
Journal	Industrial and Engineering Chemistry Research
Volume	54
Issue number	14
DOIs	https://doi.org/10.1021/ie504438z
State	Published - 15 Apr 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015 American Chemical Society.

ASJC Scopus subject areas

General Chemistry
General Chemical Engineering
Industrial and Manufacturing Engineering

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/ie504438z

Cite this

Karimi-Maleh, H., Tahernejad-Javazmi, F., Atar, N., Yola, M. L., Gupta, V. K., & Ensafi, A. A. (2015). A Novel DNA biosensor based on a pencil graphite electrode modified with polypyrrole/functionalized multiwalled carbon nanotubes for determination of 6-mercaptopurine anticancer drug. Industrial and Engineering Chemistry Research, 54(14), 3634-3639. https://doi.org/10.1021/ie504438z

@article{69d82ec939854bb387695c6bfda4189c,

title = "A Novel DNA biosensor based on a pencil graphite electrode modified with polypyrrole/functionalized multiwalled carbon nanotubes for determination of 6-mercaptopurine anticancer drug",

abstract = "A novel and sensitive biosensor employing immobilized DNA on a pencil graphite electrode modified with polypyrrole/functionalized multiwalled carbon nanotubes for the determination of 6-mercaptopurine (6-MP) is presented. In the first step, we modified the pencil graphite surface with polypyrrole and functionalized multiwalled carbon nanotubes (MWCNT/COOH). The developed electrode was characterized by scanning electron microscopy, atomic force microscopy, reflection-absorption infrared spectroscopy, X-ray photoelectron spectroscopy, and electrochemical impedance spectroscopy. In the other step, we used decreases in the oxidation responses of guanine and adenine as a sign of the interaction of 6-MP with salmon sperm double-stranded DNA using differential pulse voltammetry. The signal of guanine oxidation was linear with respect to the 6-MP concentration in the range of 0.2-100 μmol L-1 with a detection limit of 0.08 μmol L-1. The modified electrode was utilized for the determination of 6-MP in real samples.",

author = "Hassan Karimi-Maleh and Fahimeh Tahernejad-Javazmi and Necip Atar and Yola, \{Mehmet L{\"u}tfi\} and Gupta, \{Vinod Kumar\} and Ensafi, \{Ali A.\}",

note = "Publisher Copyright: {\textcopyright} 2015 American Chemical Society.",

year = "2015",

month = apr,

day = "15",

doi = "10.1021/ie504438z",

language = "English",

volume = "54",

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Karimi-Maleh, H, Tahernejad-Javazmi, F, Atar, N, Yola, ML, Gupta, VK & Ensafi, AA 2015, 'A Novel DNA biosensor based on a pencil graphite electrode modified with polypyrrole/functionalized multiwalled carbon nanotubes for determination of 6-mercaptopurine anticancer drug', Industrial and Engineering Chemistry Research, vol. 54, no. 14, pp. 3634-3639. https://doi.org/10.1021/ie504438z

A Novel DNA biosensor based on a pencil graphite electrode modified with polypyrrole/functionalized multiwalled carbon nanotubes for determination of 6-mercaptopurine anticancer drug. / Karimi-Maleh, Hassan; Tahernejad-Javazmi, Fahimeh; Atar, Necip et al.
In: Industrial and Engineering Chemistry Research, Vol. 54, No. 14, 15.04.2015, p. 3634-3639.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

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AU - Karimi-Maleh, Hassan

AU - Tahernejad-Javazmi, Fahimeh

AU - Atar, Necip

AU - Yola, Mehmet Lütfi

AU - Gupta, Vinod Kumar

AU - Ensafi, Ali A.

PY - 2015/4/15

Y1 - 2015/4/15

N2 - A novel and sensitive biosensor employing immobilized DNA on a pencil graphite electrode modified with polypyrrole/functionalized multiwalled carbon nanotubes for the determination of 6-mercaptopurine (6-MP) is presented. In the first step, we modified the pencil graphite surface with polypyrrole and functionalized multiwalled carbon nanotubes (MWCNT/COOH). The developed electrode was characterized by scanning electron microscopy, atomic force microscopy, reflection-absorption infrared spectroscopy, X-ray photoelectron spectroscopy, and electrochemical impedance spectroscopy. In the other step, we used decreases in the oxidation responses of guanine and adenine as a sign of the interaction of 6-MP with salmon sperm double-stranded DNA using differential pulse voltammetry. The signal of guanine oxidation was linear with respect to the 6-MP concentration in the range of 0.2-100 μmol L-1 with a detection limit of 0.08 μmol L-1. The modified electrode was utilized for the determination of 6-MP in real samples.

AB - A novel and sensitive biosensor employing immobilized DNA on a pencil graphite electrode modified with polypyrrole/functionalized multiwalled carbon nanotubes for the determination of 6-mercaptopurine (6-MP) is presented. In the first step, we modified the pencil graphite surface with polypyrrole and functionalized multiwalled carbon nanotubes (MWCNT/COOH). The developed electrode was characterized by scanning electron microscopy, atomic force microscopy, reflection-absorption infrared spectroscopy, X-ray photoelectron spectroscopy, and electrochemical impedance spectroscopy. In the other step, we used decreases in the oxidation responses of guanine and adenine as a sign of the interaction of 6-MP with salmon sperm double-stranded DNA using differential pulse voltammetry. The signal of guanine oxidation was linear with respect to the 6-MP concentration in the range of 0.2-100 μmol L-1 with a detection limit of 0.08 μmol L-1. The modified electrode was utilized for the determination of 6-MP in real samples.

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A Novel DNA biosensor based on a pencil graphite electrode modified with polypyrrole/functionalized multiwalled carbon nanotubes for determination of 6-mercaptopurine anticancer drug

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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