A nanocatalyst-based assay for proteins: DNA-free ultrasensitive electrochemical detection using catalytic reduction of p-nitrophenol by gold-nanoparticle labels

Jagotamoy Das; Md Abdul Aziz; Haesik Yang

doi:10.1021/ja0672167

A nanocatalyst-based assay for proteins: DNA-free ultrasensitive electrochemical detection using catalytic reduction of p-nitrophenol by gold-nanoparticle labels

Jagotamoy Das
, Md Abdul Aziz
, Haesik Yang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

313 Scopus citations

Abstract

This communication reports a nanocatalyst-based electrochemical assay for proteins. Ultrasensitive detection has been achieved by signal amplification combined with noise reduction: the signal is amplified both by the catalytic reduction of p-nitrophenol to p-aminophenol by gold-nanocatalyst labels and by the chemical reduction of p-quinone imine to p-aminophenol by NaBH₄; the noise is reduced by employing an indium tin oxide electrode modified with a ferrocenyl-tethered dendrimer and a hydrophilic immunosensing layer.

Original language	English
Pages (from-to)	16022-16023
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	128
Issue number	50
DOIs	https://doi.org/10.1021/ja0672167
State	Published - 20 Dec 2006
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja0672167

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title = "A nanocatalyst-based assay for proteins: DNA-free ultrasensitive electrochemical detection using catalytic reduction of p-nitrophenol by gold-nanoparticle labels",

abstract = "This communication reports a nanocatalyst-based electrochemical assay for proteins. Ultrasensitive detection has been achieved by signal amplification combined with noise reduction: the signal is amplified both by the catalytic reduction of p-nitrophenol to p-aminophenol by gold-nanocatalyst labels and by the chemical reduction of p-quinone imine to p-aminophenol by NaBH4; the noise is reduced by employing an indium tin oxide electrode modified with a ferrocenyl-tethered dendrimer and a hydrophilic immunosensing layer.",

author = "Jagotamoy Das and Aziz, \{Md Abdul\} and Haesik Yang",

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A nanocatalyst-based assay for proteins: DNA-free ultrasensitive electrochemical detection using catalytic reduction of p-nitrophenol by gold-nanoparticle labels. / Das, Jagotamoy; Aziz, Md Abdul; Yang, Haesik.
In: Journal of the American Chemical Society, Vol. 128, No. 50, 20.12.2006, p. 16022-16023.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A nanocatalyst-based assay for proteins

T2 - DNA-free ultrasensitive electrochemical detection using catalytic reduction of p-nitrophenol by gold-nanoparticle labels

AU - Das, Jagotamoy

AU - Aziz, Md Abdul

AU - Yang, Haesik

PY - 2006/12/20

Y1 - 2006/12/20

N2 - This communication reports a nanocatalyst-based electrochemical assay for proteins. Ultrasensitive detection has been achieved by signal amplification combined with noise reduction: the signal is amplified both by the catalytic reduction of p-nitrophenol to p-aminophenol by gold-nanocatalyst labels and by the chemical reduction of p-quinone imine to p-aminophenol by NaBH4; the noise is reduced by employing an indium tin oxide electrode modified with a ferrocenyl-tethered dendrimer and a hydrophilic immunosensing layer.

AB - This communication reports a nanocatalyst-based electrochemical assay for proteins. Ultrasensitive detection has been achieved by signal amplification combined with noise reduction: the signal is amplified both by the catalytic reduction of p-nitrophenol to p-aminophenol by gold-nanocatalyst labels and by the chemical reduction of p-quinone imine to p-aminophenol by NaBH4; the noise is reduced by employing an indium tin oxide electrode modified with a ferrocenyl-tethered dendrimer and a hydrophilic immunosensing layer.

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DO - 10.1021/ja0672167

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VL - 128

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JF - Journal of the American Chemical Society

IS - 50

ER -

A nanocatalyst-based assay for proteins: DNA-free ultrasensitive electrochemical detection using catalytic reduction of p-nitrophenol by gold-nanoparticle labels

Abstract

ASJC Scopus subject areas

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