TY - JOUR
T1 - A liquid metal-polydopamine composite for cell culture and electro-stimulation
AU - Allioux, Francois Marie
AU - Merhebi, Salma
AU - Liu, Li
AU - Centurion, Franco
AU - Abbasi, Roozbeh
AU - Zhang, Chengchen
AU - Ireland, Jake
AU - Biazik, Joanna M.
AU - Mayyas, Mohannad
AU - Yang, Jiong
AU - Mousavi, Maedehsadat
AU - Ghasemian, Mohammad B.
AU - Tang, Jianbo
AU - Xie, Wanjie
AU - Rahim, Md Arifur
AU - Kalantar-Zadeh, Kourosh
N1 - Publisher Copyright:
© 2023 The Royal Society of Chemistry.
PY - 2023/4/4
Y1 - 2023/4/4
N2 - Gallium (Ga) is a low melting point metal in the liquid state in the biological environment which presents a unique combination of fluidity, softness, and metallic electrical and thermal properties. In this work, liquid Ga is proposed as a biocompatible electrode material for cell culture by electro-stimulation since the cytotoxicity of Ga is generally considered low and some Ga compounds have been reported to exhibit anti-bacterial and anti-inflammatory activities. Complementarily, polydopamine (PDA) was coated on liquid Ga to increase the attachment capability of cells on the liquid Ga electrode and provide enhanced biocompatibility. The liquid Ga layer could be readily painted at room temperature on a solid inert substrate, followed by the formation of a nanoscale PDA coating layer resulting in a conformable and biocompatible composite electrode. The PDA layer was shown to coordinate with Ga3+, which is sourced from liquid Ga, providing electrical conductivity in the cell culture medium. The PDA-Ga3+ composite acted as a conductive substrate for advanced electro-stimulation for cell culture methods of representative animal fibroblasts. The cell proliferation was observed to increase by ∼143% as compared to a standard glass coverslip at a low potential of 0.1 V of direct coupling stimulation. This novel PDA-Ga3+ composite has potential applications in cell culture and regenerative medicine.
AB - Gallium (Ga) is a low melting point metal in the liquid state in the biological environment which presents a unique combination of fluidity, softness, and metallic electrical and thermal properties. In this work, liquid Ga is proposed as a biocompatible electrode material for cell culture by electro-stimulation since the cytotoxicity of Ga is generally considered low and some Ga compounds have been reported to exhibit anti-bacterial and anti-inflammatory activities. Complementarily, polydopamine (PDA) was coated on liquid Ga to increase the attachment capability of cells on the liquid Ga electrode and provide enhanced biocompatibility. The liquid Ga layer could be readily painted at room temperature on a solid inert substrate, followed by the formation of a nanoscale PDA coating layer resulting in a conformable and biocompatible composite electrode. The PDA layer was shown to coordinate with Ga3+, which is sourced from liquid Ga, providing electrical conductivity in the cell culture medium. The PDA-Ga3+ composite acted as a conductive substrate for advanced electro-stimulation for cell culture methods of representative animal fibroblasts. The cell proliferation was observed to increase by ∼143% as compared to a standard glass coverslip at a low potential of 0.1 V of direct coupling stimulation. This novel PDA-Ga3+ composite has potential applications in cell culture and regenerative medicine.
UR - https://www.scopus.com/pages/publications/85153174314
U2 - 10.1039/d2tb02079c
DO - 10.1039/d2tb02079c
M3 - Article
C2 - 37067358
AN - SCOPUS:85153174314
SN - 2050-7518
VL - 11
SP - 3941
EP - 3950
JO - Journal of Materials Chemistry B
JF - Journal of Materials Chemistry B
IS - 17
ER -