A high-precision robot-aided single-cell biopsy system

Adnan Shakoor; Tao Luo; Shuxun Chen; Mingyang Xie; James K. Mills; Dong Sun

doi:10.1109/ICRA.2017.7989635

A high-precision robot-aided single-cell biopsy system

Adnan Shakoor, Tao Luo, Shuxun Chen, Mingyang Xie, James K. Mills, Dong Sun

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

14 Scopus citations

Abstract

In this paper, we present a precise robot-aided single-cell surgery system to perform single-cell biopsy for cells <25 μm in diameter. A microfluidic chip is designed to arrange upto 100 individual cells in an array. A micropipette mounted onto a 3-DOF micromanipulator and a computer mouse-operated high-precision XY stage is developed to perform high-precision and high-throughput single-cell biopsy. The system is evaluated experimentally by extracting two organelles from adherent cells patterned in a microfluidic chip. The fluorescent-labeled nucleus and mitochondria of human foreskin fibroblast cells are biopsied to demonstrate the capability of the proposed system. The survival rate of the semi-automated biopsy is 73% and 45% for mitochondrial and nucleus biopsies, respectively.

Original language	English
Title of host publication	ICRA 2017 - IEEE International Conference on Robotics and Automation
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	5397-5402
Number of pages	6
ISBN (Electronic)	9781509046331
DOIs	https://doi.org/10.1109/ICRA.2017.7989635
State	Published - 21 Jul 2017
Externally published	Yes

Publication series

Name	Proceedings - IEEE International Conference on Robotics and Automation
ISSN (Print)	1050-4729

Bibliographical note

Publisher Copyright:
© 2017 IEEE.

ASJC Scopus subject areas

Software
Control and Systems Engineering
Electrical and Electronic Engineering
Artificial Intelligence

Access to Document

10.1109/ICRA.2017.7989635

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Shakoor, A., Luo, T., Chen, S., Xie, M., Mills, J. K., & Sun, D. (2017). A high-precision robot-aided single-cell biopsy system. In ICRA 2017 - IEEE International Conference on Robotics and Automation (pp. 5397-5402). Article 7989635 (Proceedings - IEEE International Conference on Robotics and Automation). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ICRA.2017.7989635

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title = "A high-precision robot-aided single-cell biopsy system",

abstract = "In this paper, we present a precise robot-aided single-cell surgery system to perform single-cell biopsy for cells <25 μm in diameter. A microfluidic chip is designed to arrange upto 100 individual cells in an array. A micropipette mounted onto a 3-DOF micromanipulator and a computer mouse-operated high-precision XY stage is developed to perform high-precision and high-throughput single-cell biopsy. The system is evaluated experimentally by extracting two organelles from adherent cells patterned in a microfluidic chip. The fluorescent-labeled nucleus and mitochondria of human foreskin fibroblast cells are biopsied to demonstrate the capability of the proposed system. The survival rate of the semi-automated biopsy is 73\% and 45\% for mitochondrial and nucleus biopsies, respectively.",

author = "Adnan Shakoor and Tao Luo and Shuxun Chen and Mingyang Xie and Mills, \{James K.\} and Dong Sun",

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year = "2017",

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doi = "10.1109/ICRA.2017.7989635",

language = "English",

series = "Proceedings - IEEE International Conference on Robotics and Automation",

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Shakoor, A, Luo, T, Chen, S, Xie, M, Mills, JK & Sun, D 2017, A high-precision robot-aided single-cell biopsy system. in ICRA 2017 - IEEE International Conference on Robotics and Automation., 7989635, Proceedings - IEEE International Conference on Robotics and Automation, Institute of Electrical and Electronics Engineers Inc., pp. 5397-5402. https://doi.org/10.1109/ICRA.2017.7989635

A high-precision robot-aided single-cell biopsy system. / Shakoor, Adnan; Luo, Tao; Chen, Shuxun et al.
ICRA 2017 - IEEE International Conference on Robotics and Automation. Institute of Electrical and Electronics Engineers Inc., 2017. p. 5397-5402 7989635 (Proceedings - IEEE International Conference on Robotics and Automation).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Luo, Tao

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AU - Sun, Dong

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AB - In this paper, we present a precise robot-aided single-cell surgery system to perform single-cell biopsy for cells <25 μm in diameter. A microfluidic chip is designed to arrange upto 100 individual cells in an array. A micropipette mounted onto a 3-DOF micromanipulator and a computer mouse-operated high-precision XY stage is developed to perform high-precision and high-throughput single-cell biopsy. The system is evaluated experimentally by extracting two organelles from adherent cells patterned in a microfluidic chip. The fluorescent-labeled nucleus and mitochondria of human foreskin fibroblast cells are biopsied to demonstrate the capability of the proposed system. The survival rate of the semi-automated biopsy is 73% and 45% for mitochondrial and nucleus biopsies, respectively.

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A high-precision robot-aided single-cell biopsy system

Abstract

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ASJC Scopus subject areas

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