Κ-CN gene polymorphism in Nili-ravi buffalo, Achai and Sahiwal cattle of Pakistan

Abdul ghafoor; Muhammad N. Riaz; Aamer B. Zahur; Nasir Abbas; Muhammad Yousaf; Abdullah Shah; Rafaqat Ishaq; Muhammad Suleman

doi:10.1111/1471-0307.12146

Κ-CN gene polymorphism in Nili-ravi buffalo, Achai and Sahiwal cattle of Pakistan

Abdul ghafoor, Muhammad N. Riaz, Aamer B. Zahur, Nasir Abbas, Muhammad Yousaf, Abdullah Shah, Rafaqat Ishaq, Muhammad Suleman^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Kappa-casein (κ-CN) is the subtype of casein protein, an important constituent of bovine milk protein. The current study was undertaken to investigate the genetic polymorphism in κ-CN gene of Nili-ravi buffalo, Achai and Sahiwal cattle of Pakistan using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. The Nili-ravi buffalo was found to be monomorphic (genotype BB only) for κ-CN gene. Achai cattle were polymorphic for κ-CN (having three genotypes AA, AB and BB) with a frequency of 0.70, 0.18 and 0.12, respectively, while in Sahiwal cattle, both the genotypes AA and AB were found with genotypic frequencies of 0.92 and 0.08, respectively. The presence of genotype BB in Achai cattle is surprising as it is absent in most of the cattle breeds worldwide.

Original language	English
Pages (from-to)	105-110
Number of pages	6
Journal	International Journal of Dairy Technology
Volume	68
Issue number	1
DOIs	https://doi.org/10.1111/1471-0307.12146
State	Published - 1 Feb 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2014 Society of Dairy Technology.

Keywords

Achai
Nili-ravi
PCR-RFLP
Polymorphism
Sahiwal
k-CN

ASJC Scopus subject areas

Food Science
Bioengineering
Process Chemistry and Technology

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10.1111/1471-0307.12146

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title = "Κ-CN gene polymorphism in Nili-ravi buffalo, Achai and Sahiwal cattle of Pakistan",

abstract = "Kappa-casein (κ-CN) is the subtype of casein protein, an important constituent of bovine milk protein. The current study was undertaken to investigate the genetic polymorphism in κ-CN gene of Nili-ravi buffalo, Achai and Sahiwal cattle of Pakistan using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. The Nili-ravi buffalo was found to be monomorphic (genotype BB only) for κ-CN gene. Achai cattle were polymorphic for κ-CN (having three genotypes AA, AB and BB) with a frequency of 0.70, 0.18 and 0.12, respectively, while in Sahiwal cattle, both the genotypes AA and AB were found with genotypic frequencies of 0.92 and 0.08, respectively. The presence of genotype BB in Achai cattle is surprising as it is absent in most of the cattle breeds worldwide.",

keywords = "Achai, Nili-ravi, PCR-RFLP, Polymorphism, Sahiwal, k-CN",

author = "Abdul ghafoor and Riaz, {Muhammad N.} and Zahur, {Aamer B.} and Nasir Abbas and Muhammad Yousaf and Abdullah Shah and Rafaqat Ishaq and Muhammad Suleman",

note = "Publisher Copyright: {\textcopyright} 2014 Society of Dairy Technology.",

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doi = "10.1111/1471-0307.12146",

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AU - ghafoor, Abdul

AU - Riaz, Muhammad N.

AU - Zahur, Aamer B.

AU - Abbas, Nasir

AU - Yousaf, Muhammad

AU - Shah, Abdullah

AU - Ishaq, Rafaqat

AU - Suleman, Muhammad

PY - 2015/2/1

Y1 - 2015/2/1

N2 - Kappa-casein (κ-CN) is the subtype of casein protein, an important constituent of bovine milk protein. The current study was undertaken to investigate the genetic polymorphism in κ-CN gene of Nili-ravi buffalo, Achai and Sahiwal cattle of Pakistan using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. The Nili-ravi buffalo was found to be monomorphic (genotype BB only) for κ-CN gene. Achai cattle were polymorphic for κ-CN (having three genotypes AA, AB and BB) with a frequency of 0.70, 0.18 and 0.12, respectively, while in Sahiwal cattle, both the genotypes AA and AB were found with genotypic frequencies of 0.92 and 0.08, respectively. The presence of genotype BB in Achai cattle is surprising as it is absent in most of the cattle breeds worldwide.

AB - Kappa-casein (κ-CN) is the subtype of casein protein, an important constituent of bovine milk protein. The current study was undertaken to investigate the genetic polymorphism in κ-CN gene of Nili-ravi buffalo, Achai and Sahiwal cattle of Pakistan using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. The Nili-ravi buffalo was found to be monomorphic (genotype BB only) for κ-CN gene. Achai cattle were polymorphic for κ-CN (having three genotypes AA, AB and BB) with a frequency of 0.70, 0.18 and 0.12, respectively, while in Sahiwal cattle, both the genotypes AA and AB were found with genotypic frequencies of 0.92 and 0.08, respectively. The presence of genotype BB in Achai cattle is surprising as it is absent in most of the cattle breeds worldwide.

KW - Achai

KW - Nili-ravi

KW - PCR-RFLP

KW - Polymorphism

KW - Sahiwal

KW - k-CN

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DO - 10.1111/1471-0307.12146

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VL - 68

SP - 105

EP - 110

JO - International Journal of Dairy Technology

JF - International Journal of Dairy Technology

IS - 1

ER -

Κ-CN gene polymorphism in Nili-ravi buffalo, Achai and Sahiwal cattle of Pakistan

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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