In vitro and in vivo studies of the potential anticancer gold(I) complexes bearing phosphine and thiones ligands

New derivatives of gold(I) containing the phosphines and thiones ligands having the general formula, [Au{P(t-Bu)3} (thione)]PF6, [Au2{(bis-(BPhP)methane}(Thione)2](PF6)2 and [Au2{1,1'-biphenyl-2yl-(t-Bu)2P}(phosphine)](PF6)2 will be synthesized and characterized using elemental analysis, IR, and NMR (1H, 13C, 31P) spectroscopy, ESI-MS and single crystal XRD. The in vitro cytotoxicity of these complexes as well as cisplatin will be investigated using MTT assay against a panel of cancer cell lines such as HeLa (cervical cancer cells), A549 (Lung cancer cells), MG-63(bone cancer cells) and HCT-116/ HCT-15 (colon cancer cells) as well as cisplatin-resistant cells. The most active complexes will be further tested for their in vivo activity on the nude mice, rats or other appropriate animals. In the present study, we proposed synthesis, characterizations, DNA damage and cell death studies study and the anticancer activity for the series of the potential anticancer gold/digold(I) complexes (1-15) on nude mice or other suitable animal.